Research Resources and Analytical Facility

Varian Inova 500 MHz

Manufacturer	Agilent (Santa Clara California, USA)
Specifications	3-channel Inova 500 console (1 high-band (1H/19F) channel, 2 broadband) 500 MHz Oxford magnet Varian L-700 Pulsed Gradient Driver Anti-vibration legs Variable temperature module PC with Varian LC- NMR software Gilson Liquid handler for LC-NMR
Probes	5mm TRPFG (triple resonance pulsed field gradient)-H, C, N, Inverse detection (ID, optimized for 1H) 5mm S/W PFG (switchable) 5mm ID Penta PFG (1H, 13C, 15N, 31P, and 2H) 3mm ID IFC (interchangeable Flow Cell) trpfg probe, can change from flow to tube, good for small amount samples ( 4mm Nano probe (HRMAS-High Resolution Magic Angle Spinning, for paramagnetic samples and semi-solid samples such as cells/tissues )
Software	Linux based VNMRJ
Applications	Routine 1D, 2D NMR Variable temperature experiments (-20 to +80oC) Identification and characterization small amount ( Advanced applications using 4mm nano probe for semi-solids and paramagnetic nano particle samples

NMR Policy

1. Only authorized users can have the key and access to the NMR instrument. Authorized users are defined as trained by Dr. Jianjun Chen, demonstrated capability to work on an instrument and obtained a key from campus police.
2. NO food and drinks in the NMR lab.
3. NO gloves on the keyboards.
4. NO sample preparation in the NMR room.
5. NO loose metal objects in the NMR room.
6. Users must sign in the timetable to reserve his/her time during his usage, and write in the logbook after each use. Using the instrument without signing timetable and writing log may result forfeiting his/her access to the NMR room.
7. If one signs up for a time and later find that one does not need it, one must clear his/her sign up.
8. While the department will pay for normal repair and maintenance, your supervisor will be responsible for any charge of repair due to your fault. Remember, in NMR, it is very expensive even with the smallest repair.
9. If you work on the 500 NMR with a non-regular probe (nano probe or flow probe). You must block all the time until the regular solution probe is exchanged. You must also put a sign on the keyboard warning users of the special probe. Failure to do so may result severe damage to the probe and magnet by other users, and this will immediately disqualify you working on the special probe.
10. If you notice any abnormal symptoms of the instruments, do not use it and inform Dr. Jianjun Chen/Dr. Wei Li immediately.

LCMS Policy

1. You must be trained by Dr. Jianjun Chen in order to use the LC/MS. No one is allowed to use other people’'s account unless authorized by Dr. Jianjun Chen.
2. You must reserve your time of usage and record your usage in the log book. If you just use one function (i.e. HPLC or MS), write HPLC only or MS only, so that others can use the other function. When recording your usage, write down the background ions, the solvent you use, and the ions of your compound and problems (if any) of the instrument.
3. Before you use it, check the logbook to see if the instrument is ok. If you find anything abnormal, DO NOT USE the instrument and report to Dr. Jianjun Chen immediately.
4. NO food and drinks in the LCMS lab.
5. NO gloves on the keyboards.
6. If one signs up for time and later finds that one does not need it, one must clear his/her sign up.

Policy on User Fees for Shared Instrumentation in the UT College of Pharmacy

Version 2.1 – Effective 2/1/2020

Purpose of the policy: This policy is aimed at ensuring the operation and functionally of the shared instrumentation resources within the University of Tennessee College of Pharmacy.  It limits access to specific levels of trained users and establishes user fees for long-term financial stability in the basic operation and maintenance of the equipment.

Use fee structure:

Instrument Name	Standard rate (Other UTHSC users)	Outside users
Bruker Avance III 400 MHz NMR	$40/hour	$50 per sample
Waters Xevo G2-S QTOF LCMS	$45/hour	$80 per sample
AB Sciex 4500 Triple Quad LCMS	$80/hour	$80 per sample
AB Sciex 5500 Triple Quad LCMS	$100/hour	$90 per sample
Bruker Amazon SL LCMS	$20/hour	$25 per sample
Scintillation counter	$40/hour	$60/hour
Gilson preparative HPLC	$40/hour	$50 per sample
Perkin-Elmer FT IR	$40/hour	$50 per sample
Varian Cary 1E UV	$40/hour	$50 per sample
Rudolph Autopol V Polarimeter	$40/hour	$50 per sample
Bruker Atomic Force Microscope	$75/hour§	$250/hour§

 

• Users need to acquire their own AFM probe

User access:

Access to the 400 MHz NMR, Amazon SL and smaller equipment is provided to general users with COP or UTHSC affiliation after appropriate training has been received and a user account has been established.

Access to the Xevo G2-S QTOF LCMS, the AB Sciex 4500 Triple Quad LCMS, and the AB Sciex 5500 Triple Quad LCMS is provided only to expert users with multiple years or proven experience in mass spectrometry and prior approval.

Specifics for each instrument are regulated by instrument specific policies.

Mass spectrometry specialist assisted operation:

For the Xevo G2-S QTOF LCMS, the AB Sciex 4500 Triple Quad LCMS, and the AB Sciex 5500 Triple Quad LCMS, mass spectrometry specialist assisted operation can be requested at a fee of $40/hour for COP users and $120/hour for other UTHSC users. Availability is schedule-dependent.

Fee Charges:

Monthly charges are tracked by the COP shared instrumentation manager and are provided to the business manager of the Department of Pharmaceutical Sciences. COP and other UTHSC users have to provide an account to the COP shared instrumentation manager prior to initiation of any work.

For users without current funding, the user fees need to be covered by the responsible department and usage requires prior approval by their department chair.

Prioritization:

Preference for instrument usage is given to COP and NIH major users, followed by other UTHSC users and outside users.