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Molecular Mass Determination

A dissolved analyte (protein, peptide, small molecule) is infused into a mass spectrometer using ESI-ionization. The mass to charge ratios (m/z) are measured and the molecular masses are determined using HRAM (high resolution accurate mass) MS analysis. Volatile solvents facilitating positive (proteins, peptides, small molecules) or negative (small molecules) ionization of analytes are used.

May 26, 2022