Instrumentation
Mass Spectrometer
Orbitrap Fusion Lumos – Thermo Fisher
- Tribrid mass spectrometer combining a Quadrupole, a Dual Linear Ion Trap, and an Orbitrap mass analyzers
- Choice of Fragmentation Types: CID, HCD, ETD, ETciD, EThcD
- Excellent Mass Resolution: 15,00-450,000 (FWHM) @ m/z200
- Excellent Mass Accuracy: Sub-ppm
- Excellent Sensitivity: Quantification of 1 attomole at CV<15%
- High Scan Rate: 20Hz
UHPLC Systems:
- Ultimate 3000-RSLCnano – Thermo Fisher
- Vanquish – Thermo Fisher
Software Packages:
- Xcalibur/SII 4.3 – Thermo Fisher
- Proteome Discoverer 3.1 – Thermo Fisher
- Compound Discoverer 2.1 – Thermo Fisher
- Tracefinder 4.1 – Thermo Fisher
- Lipidsearch 4.1 – Thermo Fisher
- PMI-Preview – Protein Metrics
- PMI-Byonic - Protein Metrics
- MaxQuant – Open Source
- Skyline – Open Source