Sample Preparation Guidelines
NEED HIGH-RED IMAGES FOR YOUR PUBLICATIONS AND GRANTS?
With the AIC's Zeiss Elyra 7 super-resolution microscope (SRM) system featuring Structured Illumination Microscopy (SIM), your lab can double the resolution of confocal microscopy with minimal but required changes in reagents without making significant changes to your staining protocols.
SAMPLE REQUIREMENTS
Coverslips must be #1.5H size thickness.
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- While #1.5 may be sufficient for some samples, the high precision options available offer a more consistent glass thickness needed for SRM. ***The AIC has samples available to new consults!
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Mounting media must have a RI of >1.4.
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- The AIC recommends the VECTASHIELD and ProLong product lines. All common fluorescent proteins and dyes are suitable for SIM, provided they are compatible with the available laser lines: 405, 488, 561, and 642nm.
- As the quality of the reconstructed SIM image is dependent on the signal-to-noise ratio, samples that have been optimized to have little background with precise fluorescent labeling will yield the best images.
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Book via the UT Health Sciences iLab website.