AIC Equipment

Zeiss Elyra 7 equipped with dual pco.edge sCMOS cameras, 4 Laser lines (405, 488, 561, 642) and 4 Objective lenses:
- EC-Plan Neofluar 10x/0.30 M27
- Plan-Aprochromat 40x/1.4 Oil DIC M27
- Plan-Aprochromat 63x/1.4 Oil DIC M27
- alpha Plan-Aprochromat 63x/1.46 Oil Corr M27
The Zeiss Elyra 7 system is capable of 2D and 3D wide-field microscopy, Lattice Structured Illumination Microscopy (SIM), Total Internal Reflectance Fluorescence Microscopy (TIRFM), and Single-Molecule Localization Microscopy (SMLM).
- Lattice SIM allows very light-efficient imaging resulting in less photodamage to cells. Key features include:
  - 2D imaging speeds up to 255 fps
  - Image up to 4 channels at a time
  - Resolution down to 120 nm laterally, 300 nm axially
SMLM utilizes photo-switchable fluorescent dyes and proteins to induce spontaneous “blinking” of single fluorophores. This allows investigation of subcellular structures and single molecules in both fixed and live cells. Key features include:
- Dual camera capability to image two different fluorophores at once
- Resolution down to 20nm laterally and 50 nm axially
View the sample preparation guide for the Elyra.

MOUNTING MEDIA

BRAND	PRODUCT NAME	CAT. NO.
Life Technologies	Prolong Gold Antifade Mountant	P36934
Life Technologies	Prolong Diamond Antifade Mountant	P38970
Life Technologies	Slowfade Gold Antifade Mountant	S36963
Life Technologies	Slowfade Diamond Antifade Mountant
Vector Laboratories	Vectashield Antifade Mounting Medium	H-1000-10
SouthernBiotech	Fluoromount-G	0100-01

1.5 COVERSLIPS/COVERGLASS

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