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AIC Equipment

Super Resolution Microscopy (SRM) Unit

  • Zeiss Elyra 7 equipped with dual pco.edge sCMOS cameras, 4 Laser lines (405, 488, 561, 642) and 4 Objective lenses:
    • EC-Plan Neofluar 10x/0.30 M27
    • Plan-Aprochromat 40x/1.4 Oil DIC M27
    • Plan-Aprochromat 63x/1.4 Oil DIC M27
    • alpha Plan-Aprochromat 63x/1.46 Oil Corr M27
  • The Zeiss Elyra 7 system is capable of 2D and 3D wide-field microscopy, Lattice Structured Illumination Microscopy (SIM), Total Internal Reflectance Fluorescence Microscopy (TIRFM), and Single-Molecule Localization Microscopy (SMLM).
    • Lattice SIM allows very light-efficient imaging resulting in less photodamage to cells. Key features include:
      • 2D imaging speeds up to 255 fps
      • Image up to 4 channels at a time
      • Resolution down to 120 nm laterally, 300 nm axially
  • SMLM utilizes photo-switchable fluorescent dyes and proteins to induce spontaneous “blinking” of single fluorophores. This allows investigation of subcellular structures and single molecules in both fixed and live cells. Key features include:
    • Dual camera capability to image two different fluorophores at once
    • Resolution down to 20nm laterally and 50 nm axially
  • View the sample preparation guide for the Elyra.
  • View the AIC's Zeiss Elyra 7 Super Resolution Microscopy (SRM) Flyer

Specific materials that will work with the Elyra

May 25, 2023