Nanostring nCounter Gene Expression Analysis Core

The J.S. and Bobbi Allen Gene Expression Analysis Laboratory is located in the CRB room 171 and is a fee-for-service facility.

The facility includes a Nanostring nCounter gene expression analysis system, which is designed to provide an ultrasensitive, reproducible and highly multiplexed method for detecting gene expression across all levels of biological expression. This assay provides a method for direct labeling of mRNAs and miRNAs with molecular barcodes called nCounter Reporter Probes without the use of reverse transcription or amplification. The assay can be run on total RNA isolated from any source, including formalin-fixed paraffin embedded (FFPE) samples.

For further information on the capabilities on the instrument and technology please go to the NANOSTRING website.

Services and Rates

For microRNA

For internal users, the total cost is $3360 for analysis of 12 samples on a human miRNA V2 assay ($280 per sample), which includes a $50 per sample processing fee. For external users, the total cost is $3480 for analysis of 12 samples on a human miRNA V2 assay ($290 per sample), which includes a $60 per sample processing fee.

For mRNA

There is $20 (internal user) and $25 (external user) per sample processing fee plus the cost of the kit. The cost of kits for analysis of 12 samples varies. For example, the total cost for human cancer-related gene panel kit (230 genes) is $2200 for 12 samples for UTHSC investigators. The total cost for human kinase gene panel kit (500 kinase genes) is $2900 for 12 samples for UTHSC investigators.

For example, the total cost for human cancer-related gene panel kit (230 genes) is $2260 for 12 samples for external users. The total cost for human kinase gene panel kit (500 kinase genes) is $2960 for 12 samples for external users.

Please contact L. Pfeffer for the total cost of other kits.

Contact Us

Recharge Core Director

Lawrence Pfeffer, Ph.D.
Professor of Pathology.
Room 156 CRB, 448-7855.

Dr. Pfeffer is responsible for maintaining the facility, and training and supervising users.

Operations

The following people are responsible for running the tests:

Chuan He Yang, Ph.D.
448-5017, Rm 160 CRB

Susan Pfeffer, Ph.D.
448-7121, Rm 160

Ziyun Du, Ph.D.
448-5017, Rm 158 CRB