Quantitative Real-time PCR
The LightCycler® 480 Instrument is a rapid, thermal block cycler with integrated real-time, online detection capabilities.
The BioMark™ System produces high quality data from RNA, miRNA, and DNA. Will perform real-time PCR analysis on up to 96 unique samples and 96 genes.
Universal ProbeLibrary assays are compatible with all real-time PCR instruments capable of detecting fluorescein, FITC, FAM, and/or SYBR Green I, and follow standard cycling protocols for hydrolysis probe assays.
|Roche LC480||Fluidigm Biomark|
|Format||96 or 384 well plates||12 samples x12 genes; 48 samples x 48 genes (Real-Time or SNP genotyping); 96 samples x 96 genes|
|Applications||Taqman, Sybr Green, HRM, Genotyping||Taqman, Sybr Green, Genotyping, Digital PCR|
|Advantages||Flexible format||High throughput; uses very low quantities|
|Disadvantages||Expensive for high throughput experiments||Fixed formats; samples must be pre-amplified.|
|Sample Preparation Suggestions||Eppendorf epMotion robot available for 384 well plates; samples should be in triplicate. Pre-qualified assays recommended, reference gene should be determined empirically||More than 1 technical replicate for each sample recommended.|
|Roche LC480 Prices|
|96 well Plates||$6.50|
|384 Well Plates||$7.00|
|LC480 Roche Sybr Green||$47|
|LC480 Roche Probe Master Mix||$41|
|LC480 Genotyping Master Mix||$241|
|LC480 High Resolution Melting||$345|
|Roche 1st strand cDNA synthesis Kit||$164|
|UPL Probe (100 rxn)||$8|
|LC480 use (per time slot)||$5|
|Control Gene Primers||$0|
|Fluidigm Biomark Prices|
|Gene Expression 12 samples (chip)||Contact Us|
|Gene Expression 48 samples (chip)||$290|
|Gene Expression 96 samples (chip)||$910|
|Digital PCR (chip)||Contact Us|
|Genotyping 12 samples (chip)||Contact Us|
|Genotyping 48 samples (chip)||$290|
|Genotyping 96 samples (chip)||$590|
|Prices don’t include pre-amplification, and other reagents for real-time|
Pricing for UT academics, other academics add 10%, and non-academics add 20%.