Next Generation Sequencing

ion proton

Next Generation Sequencing Request Form

For pricing and experimental design, contact Bill Taylor. For sample handling, contact Caitlin Costelle.

  314 Chip 316 Chip 318 chip P1 Chip
Coverage 300K-550K reads 1-3 Million reads 2-5 Million reads 60-90 Million reads
Data File Size 30-100 MB 300 Mb-1 GB 500 Mb-2 GB ~15 GB
# samples # samples can be barcoded depends on coverage required
RNA Seq   2 µg of DNase treated RNA, for low input samples contact us
chIPseq 1.5 ng-30 ng of chIP DNA, for low input samples contact us
miRNAseq 2 µg of enriched miRNA or 3 µg of total RNA
Platform used will depend on the number of samples submitted.
Targeted Seq 200 ng-1 µg genomic DNA, platform used will depend on the number of samples.
Exomes   2 µg of genomic DNA
Whole Genome Sequencing   500 ng-2 µg genomic DNA, for low input contact us
Special Instructions Prior arrangements must be made for data analysis; RNA samples must be DNase treated before submitting; Samples must be quantified by fluorescence.
All RNA samples must have a RIN of 8 or better from Agilent Bioanalyzer, for low input samples contact us.