Imaging Center Services
Tissue Processing (Embedding & Sectioning)
Reichert Jung UltraCut E Microtome
Tissue can be embedded is Spurr's resin, Epon, or Epon/Araldite blends, as well as LRWhite for immunocytochemistry. This lab has experience processing many types of tissue, tissue slices, tissue cultures, and pellets. A variety of embedment methods are available, such as Beem or gelatin capsules, flat molds, and flat embedment.
Thin sections, ranging from 50nm to 200nm in thickness, are routinely cut on the Reichert Jung UltraCut E microtome. Many types of grids are available to accommodate the investigators needs.
Semi-thin sections, also called thick sections, are readily cut on the Reichert Jung UltraCut E microtome, using glass knives. Counter-staining with Toluidine Blue is available.
Location and Scheduling
Located on the 3rd floor of the Link building. Please visit the web calendar to schedule equipment use. New users, please contact T.J. Hollingsworth at 448-5976 or by firstname.lastname@example.org for instructions and signup.
Negative staining can be done in the lab using uranyl acetate or phosphotungstic acid and viewed immediately on the electron microscope.
Liquid Nitrogen Availability
Two large tanks of liquid nitrogen are on hand for those investigators who need it on the occasional basis.
For questions contact:
Phone: (901) 448-5976
University of Tennessee Health Science Center
875 Monroe Ave, Suite 426
Memphis, TN 38163
Phone: (901) 448-5960
Fax: (901) 448-4685
311 Suite Link Building
William E. Armstrong, Ph.D.
Anton J. Reiner, Ph.D.
Brandy Fleming, M.S.