Imaging Center & Microtomy Core

The Neuroscience Imaging Center is located on the third floor of the Link building. It provides services in light and electron microscopy to faculty, staff and graduate students at the University of Tennessee, as well as the Memphis research community and private institutions nationwide.

Operating on a cost recovery basis, it offers use of equipment and technical services in the areas of

  • transmission electron microscopy
  • confocal microscopy
  • 3-dimensional neuron tracing and mapping
  • conventional light microscopy applications
  • tissue preparation

Available »

Transmission Electron Microscopy

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Immunogold localization of neurophysins in dense core granules of vasopressin and oxytocin nerve terminals. Courtesy Dr. William E. Armstrong

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Immunogold staining for GABA axodendritic synapses. Courtesy Dr. William E. Armstrong

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Pricing Information

For questions contact:

Amanda Preston
Technical Manager
Phone: (901) 448-5976
Email: apresto9@uthsc.edu


Contact Us

Neuroscience Institute
University of Tennessee Health Science Center
875 Monroe Ave, Suite 426
Memphis, TN 38163
Phone: (901) 448-5960
Fax: (901) 448-4685

Physical Address
426 Wittenborg Anatomy Building

Director:
William E. Armstrong, Ph.D.

Co-Director:
Anton J. Reiner, Ph.D.

Administrative Services Assistant:
Shannon Guyot

Website:
Brandy Fleming, M.S.

The Neuroscience Institute - University of Tennessee Health Science Center

The Neuroscience Institute on Facebook

Confocal Microscopy

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Rat hypothalamo-neurohypophysial system. Localization of oxytocin (red) and vasopressin neurons and axons. Courtesy Dr. William E. Armstrong

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Colocalization of GFAP (red) and SK3 channels (green). Overlap is yellow. Courtesy Dr. William E. Armstrong

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Confocal, immunofluorescence image of potassium channel subunits Kv2.1 (red) and Kv 2.2 (green). Courtesy Dr. William E. Armstrong

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Confocal microscopic image of GABA-containing terminals (red) in close apposition to oxytocin neurons (green) in rat hypothalamus. Courtesy Dr. William E. Armstrong

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From Deng and Elberger, Anatomy and Embryology 204:437-453, 2001.

Visualizing the spatial overlap of developing pathways in embryonic mouse brain using fluorescent dyes and confocal laser scanning microscopy. Courtesy Dr. Andrea J. Elberger

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Pathfinding photos From Deng and Elberger, Anatomy and Embryology, 207:177-192, 2003.

Overlapping distributions of corticothalamic and thalamocortical pathways in the early postnatal mouse visualized with confocal laser scanning microscopy. Courtesy Dr. Andrea J. Elberger

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From Deng and Elberger, Anatomy and Embryology 204:437-453, 2001.

Neuronal and glial distribution overlap in an early postnatal mouse brain visualized with confocal laser scanning microscopy. Courtesy Dr. Andrea J. Elberger

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From Deng and Elberger, Anatomy and Embryology 204:437-453, 2001.

Neurofilament distribution in the embryonic mouse brain visualized with confocal laser scanning microscopy. Courtesy Dr. Andrea J. Elberger

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From Deng and Elberger, Developmental Brain Research, 144:135-150, 2003.

The effects of moderate prenatal alcohol exposure on corpus callosum projection neurons in the early postnatal mouse visualized with confocal laser scanning microscopy. Courtesy Dr. Andrea J. Elberger

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Courtesy Dr. Larry Reiter

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Sagittal brain section of a transgenic adult mouse brain showing GAD65,67-EGFP neurons (green) and dopamine neurons stained for tyrosine hydroxlyase (red). Courtesy Dr. Fu-Ming Zhou

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Somatosensory cortical area pyramidal neurons and their apical dendrites labeled with yellow fluorescent protein.Image was taken with a 40X oil objective. Courtesy Dr. Fu-Ming Zhou

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A segment of an apical dendrite of a cortical pyramidal neuron labeled with yellow fluorescent protein. Note the numerous dendritic spines. Image was taken with a 40X oil objective. Courtesy Dr. Fu-Ming Zhou


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Dense hippocampal CA1 area pyramidal neurons and their downward-pointing apical dendrites labeled with yellow fluorescent protein. Image was taken with a 40X oil objective. Courtesy Dr. Fu-Ming Zhou


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Mouse cerebellar Purkinje cells labeled by GAD1-controlled GFP. Image was taken with a 20X objective. Courtesy Dr. Fu-Ming Zhou

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Drosophila 3rd instar larval neuromuscular junction stained with DLG (Disks Large) to identify synaptic boutons and Nc-82 (Bruchpilot) to identify syaptic active zones.Courtesy Dr. Larry Reiter

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Neuron Mapping & 3-D Tracing

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Courtesy Microbright Field, Inc.

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Courtesy Microbright Field, Inc.

Pyramidal neurons in the cerebral cortex

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Courtesy Dr. Fu-Ming Zhou

Mouse hippocampal neurons labeled with GFP. Imaged with a 20X objective on Zeiss 710

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Courtesy Dr. Fu-Ming Zhou

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