Welcome to Flow Cytometry and Cell Sorting

Facility Overview

Flow Cytometry FacilityWhat:UTHSC Flow Cytometry and Cell Sorting Laboratory. The Laboratory provides access to state-of-the-art analytical flow cytometry and cell sorting for UTHSC and Memphis area researchers. The instruments available are a BD Biosciences FACSAria Cell Sorter, a BD Biosciences LSR II Flow Cytometer, and a BD Biosciences FACSCalibur Flow Cytometer.

Who:The Administrative/Faculty Director of the Laboratory is Dr. Tony Marion, Department of Molecular Sciences. The Director of Operations is Dan Rosson, Ph.D. Dr. Rosson is the contact person for access and scheduling of services provided by the laboratory that are available to researchers at UTHSC as well as the Memphis area research community.

Where:The facilities are located in Rm. 214, Molecular Sciences Building, 858 Madison and Rm. 146, The Cancer Research Building, 19 South Manassas, both within the UTHSC campus. The LSR II flow cytometer and FACSAria cell sorter are in MSB214. The FACSCalibur flow cytometer is in Rm. 146, Cancer Research Building.

How:Individuals wishing to schedule training, use of either of the flow cytometers, operator-assisted flow cytometry, or cell sorting should contact Dr. Rosson, MSB214, 448-4279, drosson@uthsc.edu. Researchers may choose to collect flow cytometry data themselves on either the FACSCalibur or LSR II. Only operator-assisted flow cytometry and cell sorting is accessible with the FACSAria. Users of the FACSCalibur and the LSR II must first complete a two-hour training course with Dr. Rosson or provide documented evidence of training and complete familiarity with the relevant instrument's operation. Training, access to the flow cytometers, and scheduling for cell sorting or operator-assisted analysis requires an account number to which all charges may be submitted for payment.

When:Cell sorting and flow cytometry services and facilities are available Mon-Fri 9:00 AM - 5:00 PM and at other times by special request.