Nanodrop, Qubit, and SpectraMax M2e Plate Reader

 

For Nanodrop, contact MRC - (901) 448-6191
For Qubit - Lorne Rose
For SpectraMax M2e Plate reader - Lorne Rose

	Nanodrop	Qubit	SpectraMax M2e Plate Reader
Application	Absorbance Concentration of Nucleic Acid	Fluorescence Concentration of RNA or DNA	Absorbance, Florescence, Luminescence RNA, DNA, Proteins
Range	10ng-3000 ng (Accuracy diminishes less than 20 ng/µl and above 1500 ng/µl)	RNA Broad Range: 20-1000 ng RNA: 5-100 ng DNA Broad Range: 2-1000 ng DNA HS: 0.2-100 ng	Depends on the assay type
Advantages	A260/A280 ratios and A260/A230 ratios; Able to read 1 sample or 8 samples at a time	Usually not affected by organics, High accuracy and sensitivity	Able to read both 96-well plates and cuvette. Flexible platform; able to read nucleic acids and proteins. Very sensitive. Reads top and bottom, and has thermal regulation from 4°C to 45°C.
Disadvantages	Concentrations affected by contaminants	Can't tell purity of sample	Purity of samples can affect readings
Special Instructions	Samples should be in water or low TE. Nucleic acid samples only. Contaminated samples will need to be purified.	Needs ~15 min preparation time	First time users should be trained on software. MRC does not provide cuvette or plates. For kinetic assay, please write down the start and finish time.