Quantitative Real-time PCR
Universal ProbeLibrary assays are compatible with all real-time PCR instruments capable of detecting fluorescein, FITC, FAM, and/or SYBR Green I, and follow standard cycling protocols for hydrolysis probe assays.
The LightCycler® 480 Instrument is a rapid, thermal block cycler with integrated real-time, online detection capabilities.
The BioMark™ System produces high quality data from RNA, miRNA, and DNA. Will perform real-time PCR analysis on up to 96 unique samples and 96 genes.
|Roche LC480||Fluidigm Biomark|
|Format||96 or 384 well plates||12 samples x12 genes; 48 samples x 48 genes (Real-Time or SNP genotyping); 96 samples x 96 genes|
|Applications||Taqman, Sybr Green, HRM, Genotyping||Taqman, Sybr Green, Genotyping, Digital PCR|
|Advantages||Flexible format||High throughput; uses very low quantities|
|Disadvantages||Expensive for high throughput experiments||Fixed formats; samples must be pre-amplified.|
|Sample Preparation Suggestions||Eppendorf epMotion robot available for 384 well plates; samples should be in triplicate. Pre-qualified assays recommended, reference gene should be determined empirically||More than 1 technical replicate for each sample recommended.|