Next Generation Sequencing
|314 Chip||316 Chip||318 chip||P1 Chip|
|Coverage||300K-550K reads||1-3 Million reads||2-5 Million reads||60-90 Million reads|
|Data File Size||30-100 MB||300 Mb-1 GB||500 Mb-2 GB||~15 GB|
|# samples||# samples can be barcoded depends on coverage required|
|RNA Seq||2 µg of DNase treated RNA, for low input samples contact us|
|chIPseq||1.5 ng-30 ng of chIP DNA, for low input samples contact us|
|miRNAseq||2 µg of enriched miRNA or 3 µg of total RNA
Platform used will depend on the number of samples submitted.
|Targeted Seq||200 ng-1 µg genomic DNA, platform used will depend on the number of samples.|
|Exomes||2 µg of genomic DNA|
|Whole Genome Sequencing||500 ng-2 µg genomic DNA, for low input contact us|
|Special Instructions||Prior arrangements must be made for data analysis; RNA samples must be DNase treated
before submitting; Samples must be quantified by fluorescence.
All RNA samples must have a RIN of 8 or better from Agilent Bioanalyzer, for low input samples contact us.