Susan E. Senogles, Ph.D.

Susan E. Senogles, Ph.D.

Professor
Department of Microbiology, Immunology and Biochemistry


The University of Tennessee Health Science Center
858 Madison Avenue
Memphis, TN 38163
Phone: (901) 448-7077
Fax: (901) 448-7360
Email: Susan E. Senogles



Education

  • Ph.D. Institution: University of Minnesota, Department of Biochemistry
  • Postdoctoral: Duke University, Department of Cell Biology (previously Physiology)

Research Interests

Receptors of the seven transmembrane spanning domain family, such as the receptors for dopamine and epinephrine, signal to multiple effector systems through a large family of highly homologous G proteins. The main focus of my laboratory is the biochemistry of signal transduction, with particular emphasis on the biochemical mechanisms which govern signalling fidelity. The D2 dopamine receptor appears to function by activation of a complex set of intracellular signals, including the inhibition of adenylyl cyclase, activation of potassium channels and inhibition of voltage-gated calcium channels. One useful system for the study of D2 dopamine receptor signalling is the anterior lobe of the pituitary. Dopamine secreted into the hypophyseal portal circulation by hypothalamic neurons mediates the release of prolactin from the anterior lobe of the pituitary. We use several clonal cell lines which are derived from pituitary tumors but lack the D2 dopamine receptor. These cell lines allow us to apply protein mutagenesis and transfection techniques to define regions of the receptor which dictate the specificity of G protein interactions. We also are using several protein mutagenesis strategies to define the specificity of G protein coupling to a given effector, as well as defining domains on the G proteins which are specific for both receptor and effector interaction.

Representative Publications